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University of Connecticut Health Center - Know Better Care The Gregory P. Mullen NMR Structural Biology Facility & Biophysical Core Facility

Fluorescence Spectroscopy


Hitachi F-2500 Fluorescence Spectrophotometer

This is a walk-up instrument or samples can be dropped off to be run for an additional fee. Training on the instrument is also available. Scheduling of this instrument is done through the FACES ( scheduling system. See the Calendars and Scheduling page for more information. Visit the Research Store for current pricing.

Proteins with TRP residues, will emit light at a longer wavelength (fluorescence) after they have absorbed light. The wavelength at which the maximum fluorescence occurs is dependent on the environment of the florophore and thus fluorescence spectroscopy is a useful technique to study structural changes of proteins due to conditions or through interactions with ligands. Typically protein concentrations in the 1 - 10 uM range with a 1 ml sample volume are needed. Scattering of light from dust or other precipitates is problematic so filtering samples is often desirable.